@article { author = {Vallian, Sadeq}, title = {The Promyelocytic Leukemia Protein, PML, and Its Biological Function}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {220-230}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {The PML gene was first identified at the breakpoint region of t(15;17) chromosomal translocation in acutepromyelocytic leukemia (APL). There has been a large attention toward the elucidation of the biological function of PML in the cell. Our studies and those of others during the last decade resulted in elucidation of several fundamental biological functions for PML. These include: i) Regulation of transcription in a promoterdependent manner; ii) Suppression of growth and tumorigenisity of cells transformed by oncogenes; iii)Involvement in cell cycle progression through interaction with several key proteins involved in cell cycle regulation and apoptosis, e.g. p53 and pRb; iv) Association and mediation of the effects of a numberof viral regulatory protein via localization in the nucleus. These findings have introduced not only PML as amultifunction protein, but also opened new windows for analysis of mechanisms involved in leukemogenesisof the APL disease. The present article focused on the studies involving the elucidation of different biologicalproperties of PML.}, keywords = {Promyelocytic Leukemia Protein,Acute promyelocytic leukemia,RARα,Transcription,Cellular growth,Cell cycle}, url = {https://www.ijbiotech.com/article_6913.html}, eprint = {https://www.ijbiotech.com/article_6913_86e2fc82807bd22eba1a0430065fdcd1.pdf} } @article { author = {Ebrahimi, Mohammad and Vladimirovana Podolskaya, Svetlana and Grigorieva Gorovenko, Natalia}, title = {Genetic Polymorphisms of Glutathione S-Transferase mu1 (GSTM1) and Theta1(GSTT1) and Bronchial Asthma Susceptibility in Ukrainian Population}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {230-235}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Asthma is a chronic inflammatory disease, which involves a variety of different mediators, including reactive oxygen species (ROS). Previous studies have suggested that glutathione S-transferase (GST) genotypesmay play a role in determining susceptibility to bronchial asthma (BA), though the data are often conflicting.In this study we investigated GSTT1 and GSTM1 status in relation to BA in asthmatic patients inUkraine. To evaluate the role of GSTT1 and GSTM1 genotypes in susceptibility to BA, we conducted acase-control study of 95 cases of asthmatic patients and 253 population-based controls in Kiev city,Ukraine. All patients and control group were interviewed for information on lifestyle risk factors and DNAextracted from blood samples was used for genotyping. GSTT1 and GSTM1 genotypes were identified bymultiplex polymerases chain reaction. The frequenciesfor the GSTT1 null genotypes were 26.31% and14.12%, and for the GSTM1 null genotypes 41.50% and 50.59% among cases and controls, respectively.The GSTT1 null genotype was associated with an increased Chi-square for BA (χ² =6.12, P=0.013), butno relationship between BA and the GSTM1 null genotype was observed. Its possibility that genetic polymorphism in detoxifying enzymes may have a role in individuals susceptibility to BA.}, keywords = {Bronchial asthma (BA),Genetic polymorphism,Glutathione S-transferase T1 and M1}, url = {https://www.ijbiotech.com/article_6914.html}, eprint = {https://www.ijbiotech.com/article_6914_d82688c05bcbe9653b99228c59ddfcf5.pdf} } @article { author = {Mohandesan, Elmira and Mowla, Seyed Javad and Hojabri Noobari, Alireza and Yaghoobi, Mohammad Mehdi and Mesbah-Namin, Seyed Alireza}, title = {Extraction and Analysis of Ancient DNA from Human Remains of Masjede Kabood Burial Site}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {236-242}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Extraction and analysis of DNA from ancient remains has numerous applications in archeology and molecularevolution. However, it has become obvious that ancient DNA (aDNA) can be easily contaminated withmodern DNA, so it is crucial to detect contamination and to distinguish contaminant from authentic results.In the present study, we report the successful extraction and amplification of aDNA from 3000-3500 yearoldhuman remains excavated from Masjede kabood (Tabriz, North-West of Iran) burial site. To test theauthenticity of the extracted aDNA, we have developed a nested PCR/restriction enzyme digestionmethod for molecular sex determination of the skeletal remains, which their gender was known based on theirmorphology and belongings (Crown, Sword, Bracelet etc.). A simple and effective modified ethanol precipitation-based protocol was used for DNA extraction from 35 human skeletal remains. A segment of Homologous Amelogenin Gene (AMG), which has different alleles on X and Y-chromosomes, was amplified and analyzed. The obtained data were compared with anthropometric reports as a control for the rate of precision in aDNA analysis. The results showed that reliable aDNA can be extracted and amplified from archeologicalremains. The presented sex determination procedure could also be used as a reliable control for testing theauthenticity of aDNA results.}, keywords = {Ancient DNA,Amelogenin,Nested-PCR,Restriction enzyme digestion,Sex determination and Molecular archaeology}, url = {https://www.ijbiotech.com/article_6906.html}, eprint = {https://www.ijbiotech.com/article_6906_bc9ec457a202092b7f5b84be96228400.pdf} } @article { author = {Mashreghi, Mansour and Prosser, James I}, title = {Construction and Characterization of a Lux-Marked Phenanthrene Degrading Bacterium}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {243-249}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Construction of a luminescent microbial biosensor as a sensitive and rapid biotechnological tool for monitoringsurvival and activity of genetically engineered microorganisms (GEMs) in the contaminated environment hasbeen the main focus of this study. Four rifampicin resistant phenanthrene-degrading bacteria viz.,Commamonas testosteroni GZ38A, C. testosteroni GZ39, Pseudomonas putida GZ44 and P. stutzeri P16were made using gradient plate techniques. All resistant strains were transformed with the plasmid harboringthe mini-Tn5-tet transposon cassette, containing the luxAB genes to confer stability. C. testosteroniGZ38A and P. stutzeri P16 were successfully luxmarked in this manner. It was found that lux-markedstrains of C. testosteroni GZ38A were not able to degrade phenanthrene. Although the maximum growthrate of lux-marked strain was significantly lower (P < 0.05) than that of the wild type P. stutzeri P16, the P.stutzeri P16-luxAB4 was selected because it has the following criteria: the stability of expression of tetresistance over 180 generations, phenanthrene - degradability and high level of luminescence light outputin the absence and presence of phenanthrene. The addition of 1 litter (0.5 % v/v) n-decyl aldehydeproduced consistently high levels of luminescence at various stages of selected strain. Results clearly indicatedthat lux-marked strain was appropriately constructed and it is a novel biodegradative luminescentbiosensor which enables us to monitor the fate of a phenathrene bacterial degrader genetically or nongenetically made within a polluted environment.}, keywords = {Lux AB gene,Phenanthrene,Molecular Marker and Degradative bacteria}, url = {https://www.ijbiotech.com/article_6916.html}, eprint = {https://www.ijbiotech.com/article_6916_9e28f71a1e77eb471886d7558b3d789f.pdf} } @article { author = {Ghasemi, Fahimeh and Zomorodipour, Alireza and Shojai, Sharareh and Ataei, Fariba and Khodabandeh, Mahvash and Sanati, Mohammad Hossein}, title = {Using L-arabinose for Production of Human Growth Hormone in Escherichia coli, Studying the Processing of gIII::hGH Precursor}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {250-260}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Periplasmic expression of human growth hormone (hGH) in an arabinose-regulated Escherichia coli systemwas studied, using two forms of gIII signal sequence differing from each other at position 17 (carryingeither His17 or Arg17 at position -2 in the hGH precursor). The expression of hGH by the two recombinantplasmids was studied in the Top10 strain of E.coli. Results obtained from the expression analysis showed that the hGH expression in both of the recombinant bacteria are tightly regulated with arabinose. An optimal expression was found to occur in the medium containing 1.33 mM L-arabinose at 37°C. However, periplasmic expression of hGH occurs when the native signal sequence (His17-gIII) is applied. In the case of the bacteria carrying plasmid with the Arg17-gIII signal peptide, although the expression level was high, no mature protein could be detected under conditions tested. These data suggest for a probable effect of the -2 position in processing of the preprotein (gIII::hGH). Optimized growth and inducing conditions of the selected clone were investigated and application of a suitable signal peptide cleavage site for more efficient periplasmic production of hGH is discussed. The two recombinant plasmids presented in this work, have provided tools to study an aspect of amino acid sequence in the cleavage region on the processing and secretion of hGH.}, keywords = {Recombinant human Growth Hormone (rhGH),Arabinose,Periplasmic Expression,Signal peptide cleavage site}, url = {https://www.ijbiotech.com/article_6917.html}, eprint = {https://www.ijbiotech.com/article_6917_93dbf27e4fa9886a7e8f7d2bdf40735c.pdf} } @article { author = {Nosrati, Mohsen and Shojaosadati, Seyed Abbas and Sreekrishnan, Trichur Ramaswamy and Mukhopadhyay, Satya Narayan}, title = {Inhibition of Thermophilic Anaerobic Digestion of Waste Food by Long Chain Fatty Acids and Propionate}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {261-268}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Different initial concentrations of slurry waste food (discarded food), with and without the overlying layerof fat derived from the waste were anaerobically digested at 55°C. At solids concentrations less than 20g/l no significant difference was observed in terms of volatile fatty acids and methane production betweensamples containing fat layer and samples without it. However, at higher concentrations, differencesbecame more obvious. Biogas released from a 50 g/l fat excluded sample was around 100% more than thegas generated from the fat included sample with the same initial solids concentration. Inhibition by propionatewas not significant in concentrations less than 2000 mg/l. In the absence of fats, the inhibition causedby accumulation of propionate could be overcome partially by the methanogenic bacteria. Based on theenergy generated in the form of methane, it was found that thermophilic anaerobic digestion of waste foodcould be an autothermal process for fat excluded feeds.}, keywords = {Thermophilic anaerobic digestion,Waste food,Volatile fatty acids,Biogas}, url = {https://www.ijbiotech.com/article_6918.html}, eprint = {https://www.ijbiotech.com/article_6918_6dc24c14d14d2f365140dc1a58ea8f48.pdf} } @article { author = {Haddad, Raheem and Morris, Karl and Buchanan-Wollaston, Vicky}, title = {Expression Analysis of Genes Related to Oxidative Protection During Senescence in Brassica napus}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {269-278}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Leaf senescence is the sequence of events leading to cellular disassembly and mobilization of releasedmaterials that cause accumulation of reactive oxygen species. DNA sequencing of one of senescence-isolatedcDNA, LSC650, showed a high level of similarity with a catalase gene of Arabidopsis. Transcript level ofthis isoform was highly increased during senescence. The enzyme activity of catalase was also assayed duringdifferent developmental stages in Brassica napus and showed a high level of activity during last part ofleaf senescence. Both kinetic assay and northern analysis exhibit that catalase has the potential to playa significant role in the cell defense against produced hydrogen peroxide as part of the macromoleculesbreakdown. Gene expression pattern was characterized by mRNA hybridization with several antioxidantcDNA clones. Transcript levels of discussed genes were markedly increased during senescence stages.We conclude that the major part of the mRNA changes observed during senescence stages of leaves is connected with leaf senescence, whereas free radicalrelated transcripts appear to protect cells from oxidativedamage.}, keywords = {Active oxygen species,free radicals,Transcription,Northern analysis,Leaf senescence,Brassica napus}, url = {https://www.ijbiotech.com/article_6919.html}, eprint = {https://www.ijbiotech.com/article_6919_150c3b834863056808c99363641f68c3.pdf} } @article { author = {Rauf, Saeed and Rahman, Hafeez-ur- and Manzoor Khan, Tariq}, title = {Effect of Kinetin on Multiple Shoot Induction in Cotton (Gossypium hirsutum L.) cv. NIAB-999}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {279-282}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Multiple shoot induction was studied in upland cotton cv. NIAB-999. Cotyledonary nodes obtained fromaseptically raised seedling were cultured on modified Murashige and Skoog medium (MS) supplementedwith different doses of Kinetin. Cotyledonary nodes produced maximum number of shoots (3.43 shoots/explant) when cultured on MS medium supplemented with 0.25 mgl-1 Kinetin. Highest percentage (93.3 %) ofroot development and root length (5.85 cm) was obtained when shoots were cultured on MS mediumsupplemented with 0.5 mgl-1 napthalene acetic acid (NAA) and 0.1 mgl-1 Kinetin.}, keywords = {cotton,Cotyledonary nodes,Kinetin,Multiple shoots,Shoot tip}, url = {https://www.ijbiotech.com/article_6920.html}, eprint = {https://www.ijbiotech.com/article_6920_195937a432e2058f9b87ce02eef45c84.pdf} }