eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
130
139
7015
Differential Expression of Arabidopsis thaliana Acid Phosphatases in Response to Abiotic Stresses
Tahmineh Lohrasebi
lohrasebi@nigeb.ac.ir
1
Mohammad Ali Malboobi
malboobi@nigeb.ac.ir
2
Ali Samaeian
3
Vahid Sanei
4
Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, IR Iran
Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, IR Iran
Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, IR Iran
Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, IR Iran
The objective of this research is to identify Arabidopsis thaliana genes encoding acid phosphatases induced by phosphate starvation. Multiple alignments of eukaryotic acid phosphatase amino acid sequences led to the classification of these proteins into four groups including purple acid phosphatases (PAPs). Specific primers were degenerated and designed based on conserved sequences of PAPs isolated from plants, fungi and animals. RNA profiles of Pi-fed and Pi-starved A. thaliana roots were compared with two methods established for gene-family-directed differential display of pap genes. Having analyzed the differentially displayed fragments, seven pap encoding cDNA clones were isolated. One of the clones was a trans-splicing product of two genes that encodes an acid phosphatase carrying a zinc-finger domain. Six other clones were predicted to encode secretory phosphatases. Reverse-northern blotting and semi-quantitative RT-PCR revealed distinct expression patterns for each gene under diverse environmental conditions such as Pi starvation, high-salt concentration, cold shock, nitrogen and sulfur deprivation. The presented data can provide some clues for dissecting the possible roles of PAPs in Pi acquisition.
https://www.ijbiotech.com/article_7015_1c55452adf9e8bc82d3a704c8225c336.pdf
Acid Phosphatase
Purple acid phosphatase
differential display
Gene expression
Arabidopsis thaliana
Abiotic stress
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
140
146
9416
Colchicine induced embryogenesis and doubled haploid production in maize (Zea mays L.) anther culture
Payam Pour Mohammadi
1
Ahmad Moieni
moieni_a@modares.ac.ir
2
Mokhtar Jalali-Javaran
3
Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, P.O. Box 14115-336, Tehran,
I.R. Iran
Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, P.O. Box 14115-336, Tehran,
I.R. Iran
Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, P.O. Box 14115-336, Tehran,
I.R. Iran
This study involves in vitro androgenesis of Zea mays L. via anther culture. Combination of two embryo induction media (IMSS & YPm) in presence of different colchicine concentrations (0, 100, 200, 250, 300 and 400 mg/l ) in the pretreatment medium (IML) and pretreatment duration (0, 3, 6 and 9 days) in two genotypes (DH5×DH7 and ETH-M82) were tested. After colchicine pretreatment, anthers were transferred to the induction media without colchicine to induce of embryo like structures (ELSs). The ELSs were then transferred to plant regeneration medium (YPNAS). It was found that in the genotype DH5×DH7, colchicine at a concentration of 100 mg/l significantly induced the number of ELSs (19.6). The control (without colchicine) and 400 mg/l of colchicine resulted in lower levels of ELSs (5.8, 5.7, respectively). In this genotype, colchicine pretreatment for 3 days produced highest number of ELSs (16.83) and a large increase in the ELS yield was observed in the YPm medium (14.4). In ETH-M82 genotype, 6 days of pretreatment with 300 mgl-1 with colchicine, produced highest frequency of ELSs (25). Also, in this genotype, a large increase in ELS yield was observed in the YPm induction medium (22.3). The frequency of spontaneous chromosome doubling in control group was very low for both genotypes (7%), but these genotypes were able to produce doubled haploid plantlets from the ELSs (63% doubled haploid) using a low concentration of colchicine in the pretreatment medium (250 mgl-1 for 6 days). At high concentrations of colchicine (300 and 400 mg/l), more morphological and chromosomal aberrations were observed.
https://www.ijbiotech.com/article_9416_7f9e790775cea8b25fc814a7d172e6ae.pdf
maize
Anther Culture
colchicine
embryogenesis
Doubled haploid
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
147
152
7019
Comparison of Genetic Diversity in Species and Cultivars of Pistachio (Pistacia sp. L.) Based on Amplified Fragment Length Polymorphism (AFLP) Markers
Masoud Ahmadi Afzadi
masoud_a_a@alumni.iut.ac.ir
1
Badraldin Ebrahim Sayed Tabatabaei
2
Sayed Abolghasem Mohammadi
3
Ali Tajabadipur
4
Department of Plant Breeding, Pistachio Research Institute of Iran, Rafsanjan P.O. Box 77175-435, I.R. Iran
Department of Agricultural Biotechnology, Faculty of Agriculture, Isfahan University of Technology, Isfahan P.O. Box 84156, I.R. Iran
Department of Agronomy & Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz P.O. Box 51664, I.R. Iran
Department of Plant Breeding, Pistachio Research Institute of Iran, Rafsanjan P.O. Box 77175-435, I.R. Iran
The genetic diversity of a large number of pistachio genotypes grown in Iran is not exactly known. Most of the studies on genetic diversity of Iranian pistachio varieties are based on morphological characteristics or isozyme markers. In the present study, the genetic diversity of selected pistachio cultivars along with some wild species were evaluated by Amplified Fragment Length Polymorphism (AFLP) markers. 13 AFLP primers were used for evaluation of 45 pistachio genotypes. Totally, 506 polymorphic bands with an average polymorphism of 95.2% were detected. Cluster analysis assigned pistachio genotypes into 4 groups. All commercially cultivated pistachios were clustered in group I and II. The wild pistachio genotypes were separated from the cultivated ones. These data demonstrate that AFLP is a reliable tool for analyzing pistachio genetic diversity.
https://www.ijbiotech.com/article_7019_e3287c7a36e8767c61e08a3ca3e3b42c.pdf
Pistachio
genetic diversity
Polymorphism
AFLP
Cluster analysis
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
153
157
7017
Production of Ice Nucleation Deficient (Ice-) Mutants of the Epiphytic Strains of Erwinia herbicola
Mojtaba Keikhasaber
mkeikhasaber@yahoo.com
1
Heshmatollah Rahimian
rahimian.h@gmail.com
2
Nadali Babaeian Jelodar
3
Mahmmadreza Bolouri Moghaddam
4
Department of Biotechnology, Faculty of Agriculture, Zabol university, P.O. Box 98615-538, Zabol, I.R. Iran
Department of Plant protection, Faculty of Agriculture, Mazandaran university, P.O. Box 578, Sari, I.R. Iran
Department of Plant breeding, Faculty of Agriculture, Mazandaran university, P.O. Box 578, Sari, I.R. Iran
School of Agriculture, Neyshabour university, P.O. Box 443, Neyshabour, I.R. Iran
To mutate the Ice Nucleation Active (INA) gene in Erwinia herbicola strains, Tn-5 transposon carried by Psup2021 plasmid was used. This plasmid was transferred to the bacterial cells by electroporation. Electrotransformation was carried out for 2.5 ms at 1800 v and 1 mm distance between the electrodes. Polymerase chain reaction was used for determination of presence or loss of INA gene, using a pair of primers designed for inaZ gene. The ice nucleation activity was tested by the freezing assay technique. The ice- phenotype of the putative mutants were assayed through spraying sunflower seedlings, grown under aseptic condition, with 107 to 108 colony forming units per milliliter of the parent and transformant strains. The treated seedlings were kept at -8°C to -10°C for 8-10 hours and then transferred back to ambient (laboratory) condition for recovery or expression of freezing damage syndrome. Three out of ten electroporated strains yielded ice- mutants that failed to freeze water droplets at -8°C and produce no freezing injury on sunflower seedlings.
https://www.ijbiotech.com/article_7017_83facfe30eccfda7ea77ec5b8fbc1fd8.pdf
INA
Erwinia herbicola
inaZ geneTransposon
Tn-5
Electroporation
Sunflower
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
158
161
7020
Association of Prolactin Gene Variants with Milk Production Traits in Russian Red Pied Cattle
Masoud Alipanah
alipanah.masoud@gmail.com
1
Lobov Kalashnikova
2
Genadi Rodionov
3
Department of Animal Science, Faculty of Agriculture, University of Zabol, P.O. Box 98615-538, Zabol, IR Iran
All-Russian Research Institute of Animal Breeding, P.O. Box 141212, Moscow, Russia
Department of Dairy Cattle Breeding, Faculty of animal science,Timiriazev Agricultural University, P.O. Box 127550, Moscow, Russia
A total of 125 Russian Red Pied cows were genotyped for the prolactim-related gene. The PRL-RsaI genotypes were analysed using the Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) method. In this breed, the frequencies of alleles were as follows; A= 0.794 and B= 0.206. The frequencies of AA, AB and BB genotypes were 0.598, 0.392 and 0.01; respectively. Results showed that: BB genotype had higher milk yield than AA and AB individuals (P< 0.05). BB genotype showed higher milk fat yield than AA and AB individuals (P< 0.05). With respect to milk fat content (%), the AB genotype had higher levels than the AA and BB individuals (P< 0.05). No differences between the cows of different PRL-RsaI genotypes were found in terms of milk yield and milk protein concentration. The results showed that the highest milk and milk fat yields were obtained by cows with the genotype PRL-RsaI BB. The results presented here demonstrate that the prolactin gene may be considered as a marker for dairy traits in cattle.
https://www.ijbiotech.com/article_7020_df7b942ab2fb20fd7e5729d9e88f1602.pdf
prolactin
Polymorphism
Cattle
PCR-RFLP
Milk production
Red Pied
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
162
169
7021
Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris
Ali Bahrami
1
Seyed Abbas Shojaosadati
shoja@modares.ac.ir
2
Rasoul Khalilzadeh
3
Ali Reza Saeedinia
4
Ebrahim Vasheghani Farahani
evf@modares.ac.ir
5
Jafar Mohammadian-Mosaabadi
6
Department of Chemical Engineering, Biotechnology Group, Faculty of Engineering, Tarbiat Modares University, Tehran, P.O. Box 14155-143, I.R. Iran
Department of Chemical Engineering, Biotechnology Group, Faculty of Engineering, Tarbiat Modares University, Tehran, P.O. Box 14155-143, I.R. Iran
Institute of Biotechnology, Malek Ashtar University, P.O. Box 15875-1774, Tehran, I.R. Iran
Institute of Biotechnology, Malek Ashtar University, P.O. Box 15875-1774, Tehran, I.R. Iran
Department of Chemical Engineering, Biotechnology Group, Faculty of Engineering, Tarbiat Modares University, Tehran, P.O. Box 14155-143, I.R. Iran
Institute of Biotechnology, Malek Ashtar University, P.O. Box 15875-1774, Tehran, I.R. Iran
Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as temperature and pH on the P. pastoris cell growth and hG-CSF production during fermentation were investigated. Cell growth and hG-CSF production were found to be optimal at 28°C and pH 6.0. A fed-batch fermentation process was also developed to obtain high cell density and higher levels of protein expression. Using a high cell density cultivation method, cell dry weight and hG-CSF concentration reached 100 g/l and 35 mg/l, respectively.
https://www.ijbiotech.com/article_7021_0046d351d8223494763708302c415dc7.pdf
Pichia pastoris
rhG-CSF
Fed-batch culture
Methanol
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
170
177
7022
Biobleaching of Bagasse Pulp with Xylanase Enzymes and Hydrogen Peroxide
Saeed Soltanali
1
Yaser Ziaie-Shirkolaee
ya_ziaee@yahoo.com
2
Department of Chemical Engineering, Faculty of Engineering, Tehran University, P.O Box 11365-4563, Tehran, IR Iran
Department of Chemical Engineering, Faculty of Engineering, Tehran University, P.O Box 11365-4563, Tehran, IR Iran
The effect of the operating conditions (temperature, time, pH, enzyme level and pulp consistency) used in the enzymatic step of a XP (Cartazyme-hydrogen peroxide) sequence for bleaching Kraft pulp from bagasse on various properties of the resulting pulp and paper sheet products was studied. The quality of bagasse used, was examined by its yield, brightness,viscosity and the Kappa number. Finally, the quality of paper sheets produced were examined by their brightness, breaking length, burst index and tear index. The total number of experiments required for five independent variables was calculated from N=2k+2k+1 equation, in which k is the number of independent variables. The results of 43 experiments performed, were processed using the MINITAB software suite which provided equations that reproduced the values of the dependent variables with less error. The application of the steepest ascent method has been carefully inserted in the experimental design section the identification of the most suitable conditions for optimizing the values of the dependent variables. Based on the results, using enzyme level 6 (IU/g) , temperature 35°C, pH 5 and pulp consistency 12% for 2 hrs. in the enzymatic step provided paper sheets of acceptable quality.
https://www.ijbiotech.com/article_7022_102c40465596eb2109f6c0154817d9db.pdf
Xylanase
Bagasse
Hydrogen peroxide
biobleaching
Central Composite Design (CCD)
eng
National Institute of Genetic Engineering and Biotechnology of Iran
Iranian Journal of Biotechnology
1728-3043
2322-2921
2007-07-01
5
3
178
182
7025
Bioproduction of Indole Acetic Acid by Rhizobium Strains Isolated from Root Nodules of Green Manure Crop, Sesbania sesban (L.) Merr.
Mutluru Sridevi
1
Konada Veera Mallaiah
kvmallaiah@rediffmail.com
2
Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar-522 510, Guntur (Dt.), Andhra Pradesh, India
Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar-522 510, Guntur (Dt.), Andhra Pradesh, India
Twenty six Rhizobium strains were isolated from root nodules of Sesbania sesban (L.) Merr. collected from different regions of Andhra Pradesh. All the 26 Rhizobium strains produced indole acetic acid (IAA), but maximum amount was produced by only five strains in yeast extract mannitol (YEM) medium supplemented with L-tryptophan. The strains were found to elaborate maximum IAA when fed with 2.5 mg/ml L-tryptophan. Cultural requirements were optimized for maximum growth and IAA production. The strains differ in their growth and production of IAA on different carbon and nitrogen sources. Addition of cell wall affecting agents increased the IAA production over controls. The compound was extracted, purified and structurally confirmed as IAA.
https://www.ijbiotech.com/article_7025_411f62a5058646e3b78c339c8c7ea025.pdf
IAA production
LC-MS
Rhizobium species
Sesbania sesban