2024-03-29T11:36:28Z
https://www.ijbiotech.com/?_action=export&rf=summon&issue=1163
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Transformation of Rapeseed (Brassica napus L.) Plants with Sense and Antisense Constructs of the Fatty Acid Elongase Gene
Alireza
Zebarjadi
Mokhtar
Jalali Javaran
Ghasem
Karimzadeh
Ahmad
Moeini
Amir
Mousavi
Ali Hatef
Salmanian
The biosynthetic pathways of saturated and unsaturated fatty acids consist of many steps controlled by various enzymes. One of the methods for improving oil quality is to change the fatty acid profile through genetic manipulation which requires isolation and characterization of the genes and other cis-acting elements, such as the promoter, involved in fatty acid biosynthesis. b-ketoacyl-CoA synthase (KCS) that is the key enzyme in erucic acid biosynthesis. This enzyme is involved in producing eicosanoeic (C20:1) and erucic acids (C22:1) from C18 fatty acids, and is encoded by the fatty acid elongase (FAE) gene. Specific primers were used to amplify the FAE gene and its promoter from genomic DNA by using PCR technique. The putative gene and its promoter were cloned in sense and antisense orientation into the plant expression vector (pBI121). The sense and antisense constructs of the FAE gene were transformed via Agrobacterium-mediated transformation into low erucic acid rapeseed (LEAR such as PF) and high erucic acid cultivars (HEAR such as Maplus). The transformed plants were screened on kanamycin-containing media and then analysed by PCR and Southern blotting techniques. Moreover, erucic acid content of the first generation of transgenic (T0) plants analysed with gas chromatography, showed significant changes in fatty acid composition of transgenic rapeseed plants containing sense and antisense constructs of the FAE gene.
Brassica napus
Erucic acid
b-ketoacyl-CoA synthase
Gene cloning
Fatty acid elongase gene
Transformation
2006
04
01
79
87
https://www.ijbiotech.com/article_6995_a020353cb47c2b5ed754e842a6972b5b.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
QTL mapping of heading date and plant height in Barley cross “Azumamugi”דKanto Nakate Gold”
Fahimeh
Shahinnia
Abdolmajid
Rezai
Badraldin Ebrahim
Sayed-Tabatabaei
Takao
Komatsuda
Sayed Abolghasem
Mohammadi
To identify quantitative trait loci (QTLs) controlling heading date and plant height, ninety nine F13 recombinant inbred lines (RILs) derived from barley cultivars Azumamugi × Kanto Nakate Gold cross were evaluated. The field trails were conducted at randomized complete block design with two and three replications in 2004 and 2005, respectively. Significant differences and transgrassive segregates were observed among lines for heading date and plant height. Composite interval mapping (CIM) was done based on linkage map constructed using 99 RILs and 100 markers including isozyme, morphological, STS and AFLP markers. A strong QTL controlling 26% phenotypic variation of heading date on chromosome 5HL was located near to thee07m25.3-e12m199.1 markers. The QTL had the same interval of the Sgh2 locus. Allele inherited from Azumamugi parent in this locus decreased heading date. The QTL for heading date in the map interval of ABC261-ABG055 markers on chromosome 1HL could be identical with the eam8 locus and accounted for 11% of the phenotypic variation. New QTL for plant height was detected near to uzu1 locus on chromosome 3HL, explaining 52% of the phenotypic variation. The effect of allele transmitted from Azumamugi parentin this locus decreased plant height. The QTLs identified on chromosomes 1HL, 3HL and 7HS in relation to eam8,uzu1 and dsp1 genes showed pleiotropic effects on controllingheading date and plant height.
Composite interval mapping
Hordeum vulgare L
Major genes
Pleiotropic effects
QTLs combination
2006
04
01
88
94
https://www.ijbiotech.com/article_6982_5308d031b5e181aceee831a03d66e586.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Antifungal Activity of Heterologous Expressed Chitinase 42 (Chit42) from Trichoderma atroviride PTCC5220
Mohammad Javad
Harighi
Mostafa
Motallebi
Mohammad Reza
Zamani
The cDNA from the mycoparasitic fungus Trichoderma atroviride PTCC5220 encoding a 42 kDa chitinase (Chit42) was isolated. The nucleotide sequence of the cDNA fragment as having a 1263 bp open reading frame that encodes a 421 amino acid polypeptide, and a high homology was found withother reported Chit42 belonging to the Trichoderma sp. The 22 amino acid N-terminal sequence is a putative signal peptide for the possible secretion of the protein. The protein has been expressed and secreted as a mature form in Escherichia coli BL21(DE3) using the pelB leader sequence.The E. coli strain expressed Chit42 in an active form and secreted the protein into the medium. This recombinant chitinase has been shown to have inhibitory activity on mycelial growth and also, lytic activity on the cell wall of Rhizoctonia solani (AG2-2), causal agent of root rot in sugar beet in vitro.Expressed chitinase was optimally active at pH 5 and at 40°C. It is thermally stable at 60°C for more than 120 min at pH 5.
Trichoderma atroviride
Chitinase 42
Antifungal
Activity
Heterologous expression
2006
04
01
95
103
https://www.ijbiotech.com/article_6983_0c5c8f47988fccde53961fcc1c0a2640.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Bacterial expression and purification of C1C2 domain of human factor VIII
Amir
Amiri Yekta
Alireza
Zomorodipour
Mahvash
Khodabandeh
Morteza
Daliri Chopari
Farshid
Kafilzadeh
With the aim of the production of human factor VIII antigen and its corresponding antibody an epitope coding fragment of the light-chain of hFVIII, fused to a His6-tag, was isolated and over-expressed in Escherichia coli. The over-expressed hFVIII-epitope containing peptide was confirmed by its reaction with a rabbit serum directed against native hFVIII as well as antiHis6-tag antibody. An expression level of 6.5 mg/l (of culture) of the C1C2-related peptide was estimated. The purified product was used to develop antibody in rabbit. Theimmunoblotting experiment confirmed that the rabbit polyclonal antibodies developed against the purified bacteriallyexpressed hFVIII sub-fragment, recognizes human plasmaderived FVIII. Both the produced hFVIII-related antigen andits corresponding antibody are useful in experiments usingfor detection and purification of hFVIII as well as the clinicaldiagnosis of hFVIII related disorders.
Human factor VIII light chain
C1C2 domain
E. coli expression system
2006
04
01
104
111
https://www.ijbiotech.com/article_6999_d3bad3ee24aaeec7e0a5b63ce0304d2c.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Wild type p53 gene transfer increases chemosensitivity and apoptotic response of PANC-1 pancreatic tumor cell line
Marzieh
Hagi-Sharifia Taghavi
Manouchehr
Mirshahi
Jamshid
Davoodi
The effect of p53 gene therapy on chemosensitivity and apoptotic response of PANC-1 tumor cells, which express high amount of mutant p53, to cancer chemotherapeutic agents of Etoposide and Doxorubicin was investigated. Comparison of the chemosensitivity of PANC-1 cells to its wild type p53 transfectants showed that wt-p53 expressing transfectants are more sensitive to both Etoposide and Doxorubucin. It further showed that neither the PANC1 cells nor its wild type p53 transfectants arrested at G1 in response to X-irradiation. Furthermore, treatment of both PANC-1 cells as well as its wt-p53 transfectants with etoposide resulted inapoptosis despite the difference in their p53 status, although, the number of apoptotic cells of the wt-p53 transfectants was higher compared to the control cells. This evidence reinforce the view that combining p53 gene therapy with conventional chemotherapeutic agents may yield a more beneficial response than conventional treatments alone in pancreatic tumor cells with high amount of mutant p53.
p53
Pancreatic cancer
Apoptosis
2006
04
01
112
116
https://www.ijbiotech.com/article_7001_53fa884640692186a75ec83075f8aea3.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Genetic polymorphism at MTNR1A, CAST and CAPN loci in Iranian Karakul sheep
Feridoun
Eftekhari Shahroudi
Mohammad Reza
Nassiry
Reza
Valizadh
Alireza
Heravi Moussavi
Mojtaba
Tahmoores Pour
Heydar
Ghiasi
Genotypes for melatonin receptor type 1A (MTNR1A) and Calpastatin (CAST) were determined by enzymatic digestion of PCR products and Calpain(CAPN) genotype detected by PCR-SSCP method in Iranian Karakul sheep. Blood samples were collected from 100 purebred Karakul sheep. The extraction of genomic DNA was based on guanidinium thiocyanate- silica gel method. PCR amplicons were digested with restriction enzymes MnlI and MspI for MTNR1A and CAST genes, respectively. The MTNR1A locus had two alleles with frequencies of 0.79 for (+) and 0.21 for (-) alleles. Allelic frequencies for CAST locus were 0.85 for M and 0.15 for N. In addition, Calpain had two alleles A and B with respective frequencies of 0.79 and 0.21. The observed heterozygosity values for MTNR1A, Calpastatin and Calpain locus were 0.42, 0.29 and 0.35, respectively. The X2 test confirmed the existence of Hardy-Weinberg equilibrium for the three loci in the population. The data showed a large variation in studied genes. The genetic polymorphism could be regarded as useful tool for selection programs based on marker- assisted selection between different genotypes of those loci.
MTNR1A
Calpastatin
calpain
Polymorphism
PCR-RFLP
SSCP
Karakul Sheep
2006
04
01
117
122
https://www.ijbiotech.com/article_6998_d00a4e03b8c8c8f28ce380119f0f6cf9.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Modeling and simulation of polyhydroxybutyrate production by Protomonas extorquens in fed-batch culture
Shahrokh
Shahhoseini
Elaheh
Jamalzadeh
Modeling and simulation of Polyhydroxybutyrate (PHB) production by Protomonas extorquens in fed-batch culture were conducted in this research. The fed-batch model, developed for this process, employed a kinetic model proposed by other researchers. Several kinetic models were investigated to choose the best model. The criterion for this selection was goodness of fit (δ2). Haldane kinetic model was selected since it leads to the lowest δ2. Experimental data that have been used in this study were extracted from literature. The kinetic model was then incorporated into the process model. The values of process model parameters were estimated by employing an optimization routine developed in this study and using two sets of experimental data. These data were obtained applying two different methods of fed-batch cultivation. The values of δ2 for these two methods were 2.5 and 0.0016 respectively. Finally, simulation results were compared with two other sets of experimental data to validate the model.
Modeling
Simulation
Polyhydroxybutyrate (PHB)
Fed-batch culture
Protomonas extorquens
2006
04
01
123
129
https://www.ijbiotech.com/article_7004_f40b0e62e4b3f29b9a8ba6eac36c934c.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Public knowledge and perceptions of biotechnology and genetically modified organisms in Iran
Mohammad Hasan
Sheikhha
Seyed Mehdi
Kalantar
Ali Reza
Vahidi
Maria
Faghihi
Biotechnology offers a variety of potential environmental, social and economic benefits but, it is the center of extreme public and political debate at present. A major field of discussion in biotechnology are genetically modified (GM) organisms. New medical, political, ethical and religious discussions arise over the production and consumption of these organisms. Many surveys have been conducted in industrialized countries to investigate the public perceptions regarding the risks and benefits of biotechnology, while in developingcountries hardly any studies have been done so far. The present descriptive study intends to contribute to a better understanding of public attitudes toward biotechnology and GM foods in Iran. To assess the public’s knowledge and opinions on the dangers or opportunities of genetic modification, 300 university students as a sample of educated community and 300 individuals with no university’s education as a sample of ordinary people were asked to complete a specially designed questionnaire on the risks and benefits ofbiotechnology and GM foods. This article summarizes the current situation with regard to biotechnology, with a particular focus on GM foods and discusses the results of the survey. Our results indicated that public’s knowledge about biotechnology is low in Iran and more efforts are needed to improve their understanding of different aspects of biotechnology.
Biotechnology
Genetically Modified Organisms
Ethics
Public’s knowledge
Public’s perception
2006
04
01
130
136
https://www.ijbiotech.com/article_6975_384c8dca75d43337e64adcb332afdc72.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Bactericidal effects of essential oils from clove, lavender and geranium on multi-drug resistant isolates of Pseudomonas aeruginosa
Mohaddeseh
Mahboobi
Fereshteh
Shahcheraghi
Mohammad Mehdi
Feizabadi
The inhibitory effects of essential oils including clove, lavender and geranium extracted from Eugenia caryophyllata, Lavandula officinalis and Pelargonium graveolens on multidrug resistant isolates of Pseudomonas aeruginosa were investigated. The main constituents of clove, lavander and geranium oil were eugenol (80-90%), 1,8-cineol (13%) and citronellol (45%) respectively. Clove had the most effective essential oil against P. aeruginosa. A combination consisting of clove, lavender and geranium oils at a ratio of 3:1:1showed the most inhibitory effect (32-64 μg/ml) and strong synergy with gentamicin. The essential oils from clove, lavender and geranium exhibited bactericidal activity against multi-drug resistant strains of P.aeruginosa and may be alternatives compounds against these strains in the future.
Essential oils
Clove
lavender
Geranium
Pseudomonas aeruginosa
Antimicrobial agents
2006
04
01
137
140
https://www.ijbiotech.com/article_6997_972f7bee39d685cc72997c1fa17025c6.pdf
Iranian Journal of Biotechnology
IRAN J BIOTECH
1728-3043
1728-3043
2006
4
2
Frequency of apolipoprotein E alleles in Iranian populations
Masood
Bazrgar
M
Karimi
2006
04
01
141
141
https://www.ijbiotech.com/article_7000_9a5c4accf66574a00cdf57e1ec9fe599.pdf