National Research Center for Genetic Engineering and Biotechnology, P.O. Box: 14155-6343, Tehran, I.R. Iran.
Abstract
Production and purification of human growth hormone using a simple method was studied in two recombinant Escherichia coli, D7-5 and C27-2 strains. The r-hGH was expressed in the form of inclusion body in a batch fermentation process and purified to 99% purity using a procedure based on acid precipitation of the host derived proteins and other impurities. The effect of the pH and host strain on purification of the r-hGH and efficiency of the procedure were evaluated. It was found that the optimum pH for precipitation of the host derived proteins was 4.9. The procedure was suitable for r-hGH purification from D7-5 stain but not from the other strain C27-2. The purity of > 99% and recovery of about 40% were obtained as shown by SDS-PAGE and Western blot analysis. The purified r-hGH was biologically active as judged by receptor assay with very low endotoxin content which could be suitable for therapeutic applications. This simple and cost effective production process could be useful for large scale production of recombinant hGH from specific strains.
Khodabandeh, M., Yakhchali, B., Rahimi, M., Vaseli, N., Moghaddasi Jahromi, Z., Zomorrodipour, A., Deezagi, A., Ansari Majd, S., & Sanati, M. H. (2003). Purification of Large Quantities of Biologically Active Recombinant Human Growth Hormone. Iranian Journal of Biotechnology, 1(4), 207-212.
MLA
Mahvash Khodabandeh; Bagher Yakhchali; Maryam Rahimi; Neda Vaseli; Zahra Moghaddasi Jahromi; Alireza Zomorrodipour; Abdolkhalegh Deezagi; Saeid Ansari Majd; Mohammad H Sanati. "Purification of Large Quantities of Biologically Active Recombinant Human Growth Hormone". Iranian Journal of Biotechnology, 1, 4, 2003, 207-212.
HARVARD
Khodabandeh, M., Yakhchali, B., Rahimi, M., Vaseli, N., Moghaddasi Jahromi, Z., Zomorrodipour, A., Deezagi, A., Ansari Majd, S., Sanati, M. H. (2003). 'Purification of Large Quantities of Biologically Active Recombinant Human Growth Hormone', Iranian Journal of Biotechnology, 1(4), pp. 207-212.
VANCOUVER
Khodabandeh, M., Yakhchali, B., Rahimi, M., Vaseli, N., Moghaddasi Jahromi, Z., Zomorrodipour, A., Deezagi, A., Ansari Majd, S., Sanati, M. H. Purification of Large Quantities of Biologically Active Recombinant Human Growth Hormone. Iranian Journal of Biotechnology, 2003; 1(4): 207-212.
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