Detection of NAD(P)H: quinone oxidoreductase 609C T polymorphism in blood and archival human tissues using a simple PCR method

Authors

1 National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, I.R.Iran.

2 Department of Pathology, Cancer Institute of Tehran, Tehran University of Medical Sciences, Tehran, I.R.Iran.

3 Department of Surgery, Tehran University of Medical Sciences, Tehran, I.R.Iran.

4 Genetic Clinic Vallieasr square, Tehran, I.R. Iran.

Abstract

NAD(P)H: quinone oxidoreductase (NQO1) plays an important role in detoxification of numerous endogenous
and foreign compounds. This gene has a single nucleotide polymorphism at site of codon 187
(CCT TCT). Recently, it has been demonstrated that individuals with T allele may exhibit resistance to
quinone based anticancer drugs such as mitomycin C. In the present study, a simple and feasible method was
developed for detection of NQO1 genotype. In this modified procedure, dimethylsulfoxide (DMSO) and
Triton X-100 were eliminated, also, PCR cycling conditions were modified to improve the PCR products from
blood and formalin-fixed, paraffin-embedded tissues. PCR-RFLP and DNA sequencing analysis carried out
on a limited number of blood and archival samples. It is suggested that this procedure convenient for NQO1
genotyping.

Keywords

Iranian Journal of Biotechnology
Volume 2, Issue 3
July 2004
Pages 203-206

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